Products
ERK1/2 antibody
- Product Name
- ERK1/2 antibody
- Catalogue No.
- FNab02847
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 8C11
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- mitogen-activated protein kinase 3
- Alternative Names
- ERK antibody, ERK 1 antibody, ERK1 antibody, ERK1/2 antibody, ERT2 antibody, HS44KDAP antibody, HUMKER1A antibody, Insulin stimulated MAP2 kinase antibody, MAP kinase 1 antibody, MAP kinase 3 antibody, MAP kinase isoform p44 antibody, MAPK 1 antibody, MAPK 3 antibody, MAPK3 antibody, p44 ERK1 antibody, p44 MAPK antibody, P44ERK1 antibody, P44MAPK antibody, PRKM3 antibody
- UniProt ID
- P27361
- Observed MW
- 38-44 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB：1:500-1:5000; IHC: 1:100-1:400; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02847(ERK1/2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using FNab02847(ERK1/2 Antibody) at dilution of 1:200. Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Immunofluorescent analysis of HepG2 cells using FNab02847(ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Background
- ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.
